yamamoto lab talen accessory pack (Addgene inc)

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Addgene inc yamamoto lab talen accessory pack
Yamamoto Lab Talen Accessory Pack, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yamamoto+lab+talen+accessory+pack/yamamoto+lab+talen+accessory+pack/us12359180-202-41-46
Average 90 stars, based on 1 article reviews

yamamoto lab talen accessory pack - by Bioz Stars, 2026-10

90/100 stars

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Plasmid Preparation:

Article Title: Polypeptide containing DNA-binding domain
Article Snippet: As such, by using the vector set of Example 1 and the vector library of Example 2, the TALEN expression vectors could be prepared with a repetitive fashion for every four DNA-binding modules. .. The Golden Gate reaction was performed as described in Example 2 except that pHD1-6, pNG1-6, pNIl-6, pNN1-6 contained in Golden Gate TALEN and TAL Effector Kit (Addgene) was used in place of the vector set prepared in Example 1 and that Yamamoto Lab TALEN Accessory Pack (Addgene) was used as pFUS vector for use in the reaction, to prepare a vector library. .. Using the prepared vector library, the Golden Gate reaction was performed as described in Example 3 to prepare the TALEN expression vectors.

Expressing:

Article Title: Polypeptide containing DNA-binding domain
Article Snippet: Using the prepared ptCMV vectors, the vector library prepared in Example 2, and the vectors contained in Golden Gate TALEN and TAL Effector Kit, Yamamoto Lab TALEN Accessory Pack (both Addgene), DNA-binding domains were inserted by Golden Gate procedure as shown in FIG. 1, STEP 2 to prepare TALEN expression vectors. .. Using the prepared ptCMV vectors, the vector library prepared in Example 2, and the vectors contained in Golden Gate TALEN and TAL Effector Kit, Yamamoto Lab TALEN Accessory Pack (both Addgene), DNA-binding domains were inserted by Golden Gate procedure as shown in FIG. 1, STEP 2 to prepare TALEN expression vectors. ..

Article Title: Polypeptide containing DNA-binding domain
Article Snippet: Using pTALEN v2 and pcDNA-TAL-NC2 (both Addgene), In-Fusion cloning was performed, a BsmBI site adjacent sequence for incorporating modules was prepared by In-Fusion cloning, and a globin leader sequence was introduced upstream the initiation codon by In-Fusion cloning to prepare ptCMV vectors as shown in FIG. 1, STEP 2. .. Using the prepared ptCMV vectors, the vector library prepared in Example 2, and the vectors contained in Golden Gate TALEN and TAL Effector Kit, Yamamoto Lab TALEN Accessory Pack (both Addgene), DNA-binding domains were inserted by Golden Gate procedure as shown in FIG. 1, STEP 2 to prepare TALEN expression vectors. ..

Construct:

Article Title: A mitochondrial phosphatase PTPMT1 is essential for the early development of silkworm, Bombyx mori.
Article Snippet: Juvenile hormone (JH) plays important roles in the control of many biological processes in insects, such as development, reproduction, and polyphenism.. JH is primarily produced in the corpora allata (CA) by specific JH biosynthetic enzymes under strict temporal regulation.. In a previous study, we identified a novel putative JH biosynthetic gene, protein tyrosine phosphatase, mitochondrial 1 (PTPMT1), from silkworm, Bombyx mori, whose expression is nearly exclusive in the CA and is correlated with JH synthetic activities during late larval development.

Functional Assay:

Article Title: Materials and methods for the treatment of latent viral infection
Article Snippet: .. The GG-TALEN ICP4_nt1nn pair, assembled using the Voytas Lab Golden Gate Assembly TALEN kit and Yamamoto Lab TALEN Accessory Pack (acquired via Addgene), contain RVD arrays targeted to a coding region of the ICP4 protein corresponding to a functional domain essential for ICP4′s ability to transactivate other HSV genes (see right ‘X’ in ICP4 gene, FIG. 10). ..

Article Title: Materials and methods for the treatment of latent viral infection
Article Snippet: .. The GG-TALEN ICP4_nb2nn pair, assembled using the Voytas Lab Golden Gate Assembly TALEN kit and Yamamoto Lab TALEN Accessory Pack (acquired via Addgene), contain RVD arrays targeted to a coding region of the ICP4 protein corresponding to a functional domain essential for ICP4′s DNA-binding ability (see left ‘X’ in ICP4 gene, FIG. 10). ..

other:

Article Title: Polypeptide containing DNA-binding domain
Article Snippet: Using the prepared vector library, the Golden Gate reaction was performed as described in Example 3 to prepare the TALEN expression vectors.

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